SIRT2 antibody

Catalog No. ABIN6989718

This anti-SIRT2 antibody is a Rabbit Monoclonal antibody detecting SIRT2 in IHC (p) and IF (cc). Suitable for Human, Rat, Mouse, Dog and Cow.

Quick Overview for SIRT2 antibody (ABIN6989718)

Target: SIRT2 (Sirtuin 2 (SIRT2))

Reactivity: Human, Rat, Mouse, Dog, Cow

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SIRT2 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 4G12

Product Details anti-SIRT2 Antibody

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant human SIRT2 protein, around C-terminal 150aa.

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SIRT2

Target: SIRT2 (Sirtuin 2 (SIRT2))

Alternative Name: SIRT2

Background:

Synonyms: NAD-dependent protein deacetylase sirtuin-2, SIRT2

Background: NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and alpha-tubulin as well as many other proteins such as key transcription factors. Participates in the modulation of multiple and diverse biological processes such as cell cycle control, genomic integrity, microtubule dynamics, cell differentiation, metabolic networks, and autophagy. Plays a major role in the control of cell cycle progression and genomic stability. Functions in the antephase checkpoint preventing precocious mitotic entry in response to microtubule stress agents, and hence allowing proper inheritance of chromosomes. Positively regulates the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex activity by deacetylating CDC20 and FZR1, then allowing progression through mitosis. Associates both with chromatin at transcriptional start sites (TSSs) and enhancers of active genes. Plays a role in cell cycle and chromatin compaction through epigenetic modulation of the regulation of histone H4 'Lys-20' methylation (H4K20me1) during early mitosis. Specifically deacetylates histone H4 at 'Lys-16' (H4K16ac) between the G2/M transition and metaphase enabling H4K20me1 deposition by KMT5A leading to ulterior levels of H4K20me2 and H4K20me3 deposition throughout cell cycle, and mitotic S-phase progression. Deacetylates KMT5A modulating KMT5A chromatin localization during the mitotic stress response.

Gene ID: 22933

UniProt: Q8IXJ6