ERK1/2 antibody (pThr185, pThr202)

Catalog No. ABIN6989921

The Rabbit Monoclonal anti-ERK1/2 antibody has been validated for WB, IHC, IHC (p), IF (cc) and FACS. It is suitable to detect ERK1/2 in samples from Human and Mouse.

Quick Overview for ERK1/2 antibody (pThr185, pThr202) (ABIN6989921)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ERK1/2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS)

Clone: 6H2

Product Details anti-ERK1/2 Antibody

Binding Specificity: pThr185, pThr202

Purification: Purified by Protein A.

Immunogen: Synthetic phospho-Peptide corresponding to residues surrounding Thr185 of human Erk2.

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C

Expiry Date: 12 months

Target Details for ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Alternative Name: Erk1+Erk2

Background:

The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation,differentiation, and cell cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed. [provided by RefSeq, Jul 2008].

Subcellular location: Cytoplasm, Nucleus

Gene ID: 5595

UniProt: P27361