RAC1+CDC42 (pSer71) antibody

Catalog No. ABIN6989963

The Rabbit Monoclonal anti-RAC1+CDC42 antibody is suitable to detect RAC1+CDC42 in samples from Human. It has been validated for WB, IHC, IF (cc) and IHC (p).

Quick Overview for RAC1+CDC42 (pSer71) antibody (ABIN6989963)

Target: RAC1+CDC42

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5H1

Product Details

Binding Specificity: pSer71

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: Synthetic phospho-Peptide corresponding to residues surrounding Ser71 of human CDC42.

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details

Target: RAC1+CDC42

Background:

Synonyms: CDC42Hs antibody, Cell division cycle 42 antibody, G25K antibody, MIG5 antibody, Migration inducing gene 5 antibody, p21 Rac1 antibody, Ras like protein TC25 antibody, Small GTP binding protein Cdc42 antibody, TC25 antibody

Background: The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013].

Gene ID: 998

UniProt: P60953

Pathways: WNT Signaling