BANF1 antibody (C-Term)

Catalog No. ABIN6990199

This Rabbit Polyclonal antibody specifically detects BANF1 in WB, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for BANF1 antibody (C-Term) (ABIN6990199)

Target: BANF1 (Barrier To Autointegration Factor 1 (BANF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BANF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-BANF1 Antibody

Binding Specificity: C-Term

Purification: BANF1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-BANF1 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human BANF1. The immunogen is located within the last 50 amino acids of BANF1.

Isotype: IgG

Application Details

Application Notes: WB: 0.5-2 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: BANF1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: BANF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for BANF1

Target: BANF1 (Barrier To Autointegration Factor 1 (BANF1))

Alternative Name: BANF1

Background: BANF1 Antibody: Barrier-to-autointegration factor 1 (BANF1) is a conserved chromatin protein that non-specifically binds double-stranded DNA. BANF1 also interacts with a family of nuclear proteins that include LAP2, emerin, and MAN1. It is also a host cell component of retroviral pre-integration complexes (PICs), including that of HIV. BANF1 will bind to p55 Gag (the structural precursor of HIV-1 virions) as well as its cleaved product matrix. In addition to being a host cell component of the PIC, it is thought that BANF1 is also present at low levels in incoming virions, and thus might contribute to the assembly or activity of HIV-1 PICs through direct binding to matrix as well as DNA.

Molecular Weight: Predicted: 10kD

Observed: 12 kD

Despite its predicted molecular weight, BANF1 often migrates at a higher size in SDS-PAGE. kDa

Gene ID: 8815

UniProt: O75531