ORAI1 antibody (C-Term)

Catalog No. ABIN6990201

This anti-ORAI1 antibody is a Mouse Monoclonal antibody detecting ORAI1 in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for ORAI1 antibody (C-Term) (ABIN6990201)

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ORAI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 6D11A11

Product Details anti-ORAI1 Antibody

Binding Specificity: C-Term

Specificity: ORAI1 antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

Purification: ORAI1 Monoclonal Antibody is Protein A purified.

Immunogen: Mouse monoclonal ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.

Isotype: IgG2b

Application Details

Application Notes: ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ORAI1 Monoclonal Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: ORAI1 monoclonal antibody can be stored at -20°C, stable for one year.

Target Details for ORAI1

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Alternative Name: ORAI1

Background: ORAI1 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor.

Molecular Weight: Predicted: 33 kDa

Observed: 50 kDa

Gene ID: 84876

UniProt: Q96D31

Pathways: TCR Signaling, BCR Signaling