KLF4 antibody (C-Term)

Catalog No. ABIN6990208

This Mouse Monoclonal antibody specifically detects KLF4 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for KLF4 antibody (C-Term) (ABIN6990208)

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KLF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 4G6E11

Product Details anti-KLF4 Antibody

Binding Specificity: C-Term

Specificity: At least three isoforms of KLF4 are known to exist, this antibody will detect all three. KLF4 antibody will not cross-react with other Kruppel-like family members.

Purification: KLF4 Monoclonal Antibody is affinity chromatography purified via peptide column.

Immunogen: KLF4 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human KLF4.

Isotype: IgG1

Application Details

Application Notes: KLF4 antibody can be used for detection of KLF4 by Western blot at 1 μ,g/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: KLF4 Monoclonal Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: KLF4 monoclonal antibody can be stored at -20°C, stable for one year.

Target Details for KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: KLF4

Background: KLF4 Monoclonal Antibody: KLF4 is a transcription factor that functions as both a transcriptional activator and repressor to regulate proliferation and differentiation of multiple cell types. The role of KLF4 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing KLF4 and the transcription factors POU5F1, Sox2, and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing KLF4, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. KLF4 interacts directly with POU5F1 and Sox2 in iPS and ES cells and activates the target gene NANOG.

Gene ID: 9314

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance