beta Actin antibody (C-Term) (Biotin)

Catalog No. ABIN6990218

The Mouse Monoclonal anti-beta Actin antibody has been validated for WB and ELISA. It is suitable to detect beta Actin in samples from Human, Mouse, Rat, Chicken, Rabbit, Zebrafish (Danio rerio) and Drosophila melanogaster.

Quick Overview for beta Actin antibody (C-Term) (Biotin) (ABIN6990218)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Zebrafish (Danio rerio), Drosophila melanogaster

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Clone: 10B7

Product Details anti-beta Actin Antibody (Biotin)

Binding Specificity: C-Term

Specificity: Beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.

Purification: beta-Actin Antibody is protein A purified

Immunogen: beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of beta-actin.

Isotype: IgG

Application Details

Application Notes: beta-Actin antibody can be used for detection of Beta-Actin by Western blot at 0.5 - 1 μ,/mL.

Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: beta-Actin antibody is supplied in PBS containing 1 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: beta-Actin

Background: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Molecular Weight: Predicted: 42 kDa

Observed: 42 kDa

Gene ID: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction