Vinculin antibody (N-Term)

Catalog No. ABIN6990230

The Mouse Monoclonal anti-Vinculin antibody has been validated for WB and ELISA. It is suitable to detect Vinculin in samples from Human, Mouse, Rat, Rabbit and Chicken.

Quick Overview for Vinculin antibody (N-Term) (ABIN6990230)

Target: Vinculin (VCL)

Reactivity: Human, Mouse, Rat, Rabbit, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 7E10

Product Details anti-Vinculin Antibody

Binding Specificity: AA 50-100, N-Term

Specificity: Vinculin antibody is human, mouse, rat, rabbit and chicken reactive. At least three isoforms of Vinculin are known to exist.

Purification: Vinculin antibody is purified via Protein A prification.

Immunogen: Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin. The immunogen is located within amino acids 50 - 100 of Vinculin.

Isotype: IgG

Application Details

Application Notes: Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 μ,g/mL.

Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Vinculin Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Vinculin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: Vinculin

Background: Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).

Molecular Weight: Predicted: 117 kDa

Observed: 120 kDa

Gene ID: 7414

NCBI Accession: NP_003364

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2