Vinculin antibody is human, mouse, rat, rabbit and chicken reactive. At least three isoforms of Vinculin are known to exist.
Purification
Vinculin antibody is purified via Protein A prification.
Immunogen
Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin. The immunogen is located within amino acids 50 - 100 of Vinculin.
Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).