PD-L1 antibody (Extracellular Domain)

Catalog No. ABIN6990233

The Mouse Monoclonal anti-PD-L1 antibody has been validated for WB, ELISA, IF, ICC and IHC (p). It is suitable to detect PD-L1 in samples from Human and Mouse.

Quick Overview for PD-L1 antibody (Extracellular Domain) (ABIN6990233)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 6H10

Product Details anti-PD-L1 Antibody

Binding Specificity: Extracellular Domain

Specificity: PD-L1 Antibody has no cross-reactivity to PD-L2.

Purification: PD-L1 Antibody is supplied as protein A purified IgG1.

Immunogen: Anti-PD-L1 antibody was raised against the extracellular domain of human PD-L1.

Isotype: IgG1

Application Details

Application Notes: WB: 0.25 - 4 μ,g/mL, IF: 2 μ,g/mL, IHC-P: 5 μ,g/mL, ICC: 1 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry, Immunocytochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PD-L1 Antibody is supplied in PBS containing 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PD-L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PDL1

Background: PD-L1 plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, PD-L1 modulates the activation threshold of T-cells and limits T-cell effector response (1). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (2,3). Through a yet unknown activating receptor, it may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (4).

Molecular Weight: Predicted: 33kD

Observed: 37kD kDa

Gene ID: 29126

NCBI Accession: NP_054862

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints