CCR3 antibody (C-Term)

Catalog No. ABIN6990236

The Rabbit Polyclonal anti-CCR3 antibody has been validated for WB, ELISA, IHC (p) and IF. It is suitable to detect CCR3 in samples from Human, Mouse and Rat.

Quick Overview for CCR3 antibody (C-Term) (ABIN6990236)

Target: CCR3 (Chemokine (C-C Motif) Receptor 3 (CCR3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCR3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-CCR3 Antibody

Binding Specificity: C-Term

Specificity: At least three isoforms of CCR3 are known to exist, this antibody will detect all three isoforms.

Purification: CCR3 Antibody is affinity chromatography purified via peptide column.

Immunogen: CCR3 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human CCR3. The immunogen is located within the last 50 amino acids of CCR3.

Isotype: IgG

Application Details

Application Notes: CCR3 antibody can be used for the detection of CCR3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: CCR3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: CCR3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CCR3

Target: CCR3 (Chemokine (C-C Motif) Receptor 3 (CCR3))

Alternative Name: CCR3

Background: CCR3 Antibody: Human immunodeficiency virus (HIV) and related virus require coreceptors to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b, CCR8, GPR15, STRL33, and CX3CR1 in the chemokine receptor family were recently identified as HIV coreceptors. CCR5, CXCR4 and CCR3 are the principal receptors for HIV fusion and entry of target cells. CCR3 facilitates infection by a subset of virus. CCR3 and CCR5 promote efficient infection of microglia, the major target cells in the CNS. High levels of CCR3 and CXCR4 expression were found on the neurons from both the central and peripheral nervous systems. The CCR3 ligand, eotaxin, and an anti-CCR3 antibody inhibited HIV infection of microglia. These results indicate CCR3 plays an important role in HIV infection of CNS.

Molecular Weight: Predicted: 39, 41 kDa

Observed: 52 kDa

Gene ID: 1232

NCBI Accession: NP_847899

UniProt: P51677