DAPK3 antibody (AA 270-320)

Catalog No. ABIN6990278

The Rabbit Polyclonal anti-DAPK3 antibody is suitable to detect DAPK3 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and ICC.

Quick Overview for DAPK3 antibody (AA 270-320) (ABIN6990278)

Target: DAPK3 (Death-Associated Protein Kinase 3 (DAPK3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAPK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-DAPK3 Antibody

Binding Specificity: AA 270-320

Purification: ZIPK Antibody is affinity chromatography purified via peptide column.

Immunogen: ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase. The immunogen is located within amino acids 270 - 320 of ZIPK.

Isotype: IgG

Application Details

Application Notes: ZIPK antibody can be used for detection of DNase II expression by Western blot at 1 μ,g/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ZIPK Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ZIPK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for DAPK3

Target: DAPK3 (Death-Associated Protein Kinase 3 (DAPK3))

Alternative Name: ZIPK

Background: ZIPK Antibody: Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.

Molecular Weight: Predicted: 26 kDa

Observed: 27 kDa

Gene ID: 1613

UniProt: O43293