IRAK2 antibody (C-Term)

Catalog No. ABIN6990294

This anti-IRAK2 antibody is a Rabbit Polyclonal antibody detecting IRAK2 in WB, ELISA, ICC and IF. Suitable for Human.

Quick Overview for IRAK2 antibody (C-Term) (ABIN6990294)

Target: IRAK2 (Interleukin-1 Receptor-Associated Kinase 2 (IRAK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-IRAK2 Antibody

Binding Specificity: AA 530-580, C-Term

Specificity: Anti-IRAK2 has no cross response to IRAK.

Purification: IRAK-2 Antibody is affinity chromatography purified via peptide column.

Immunogen: IRAK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IRAK2. The immunogen is located within amino acids 530 - 580 of IRAK-2.

Isotype: IgG

Application Details

Application Notes: IRAK-2 antibody can be used for detection of IRAK2 by Western blot at 2 μ,g/mL. A 65 kDa band should be detected. This polyclonal antibody can also detect IRAK2 by immunocytochemistry at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: IRAK-2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: IRAK-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for IRAK2

Target: IRAK2 (Interleukin-1 Receptor-Associated Kinase 2 (IRAK2))

Alternative Name: IRAK-2

Background: IRAK-2 Antibody: The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κ,B, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κ,B activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-κ,B activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.

Molecular Weight: 65 kDa

Gene ID: 3656

UniProt: O43187

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades