FEM1B antibody (C-Term)

Catalog No. ABIN6990338

The Rabbit Polyclonal anti-FEM1B antibody has been validated for ELISA, WB, IHC (p) and IF. It is suitable to detect FEM1B in samples from Human, Rat and Mouse.

Quick Overview for FEM1B antibody (C-Term) (ABIN6990338)

Target: FEM1B (Fem-1 Homolog B (FEM1B))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FEM1B antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-FEM1B Antibody

Binding Specificity: C-Term

Purification: F1A alpha Antibody is affinity chromatography purified via peptide column.

Immunogen: F1A antibody was raised against a 14 amino acid peptide near the carboxy terminus of human F1A. The immunogen is located within the last 50 amino acids of F1A alpha.

Isotype: IgG

Application Details

Application Notes: F1Ab antibody can be used for detection of F1Ab by Western blot at 1 μ,g/mL. An approximately 70 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse and rat samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: F1A alpha Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: F1A alpha antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for FEM1B

Target: FEM1B (Fem-1 Homolog B (FEM1B))

Alternative Name: F1A alpha

Background: F1A alpha Antibody: Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aalpha and FEM1β,. F1Aalpha/FEM1β, is the homologue of C. elegans sex determining protein FEM-1. FEM-1/F1Aalpha is cleaved by CED-3 and caspase. FEM-1/F1Aalpha associates with CED-4 and its mammalian homologue Apaf-1. Overexpression of F1Aalpha induces apoptosis. F1Aalpha is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aalpha is expressed in a variety of human and mouse tissues.

Molecular Weight: 70 kDa

Gene ID: 10116

UniProt: Q9UK73