KappaB Ras (AA 80-130) antibody

Catalog No. ABIN6990380

The Rabbit Polyclonal anti-KappaB Ras antibody is suitable to detect KappaB Ras in samples from Human, Mouse and Rat. It has been validated for ELISA, WB and IHC (p).

Quick Overview for KappaB Ras (AA 80-130) antibody (ABIN6990380)

Target: KappaB Ras

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 80-130

Purification: KappaB ras Antibody is affinity chromatography purified via peptide column.

Immunogen: KappaB ras antibody was raised against a 18 amino acid synthetic peptide from near the center of human KappaB ras 1. The immunogen is located within amino acids 80 - 130 of KappaB ras.

Isotype: IgG

Application Details

Application Notes: KappaB ras antibody can be used for detection of KappaB ras by Western blot at 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 1 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: KappaB ras Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: KappaB ras antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details

Target: KappaB Ras

Background: KappaB ras Antibody: KappaB ras-1 (κ,B-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with Iκ,B (Iκ,B), an inhibitor of the transcription factor NF-κ,B. Iκ,B exists in two homologous forms, Iκ,B-alpha and Iκ,B-beta, although Iκ,B-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive Iκ,B-alpha-NF-κ,B complexes can shuttle in and out of the nucleus, Iκ,B-beta-NF-κ,B complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the Iκ,B-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of Iκ,B-beta and regulating the rate of degradation of Iκ,B-beta. This antibody detects both kappaB-ras1 and kappaB-ras2.

Gene ID: 28512

UniProt: Q9NYS0