KappaB Ras (AA 80-130) antibody
Reactivity: Human, Mouse, Rat
ELISA, WB, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
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- Target
- KappaB Ras
- Binding Specificity
- AA 80-130
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- KappaB ras Antibody is affinity chromatography purified via peptide column.
- Immunogen
- KappaB ras antibody was raised against a 18 amino acid synthetic peptide from near the center of human KappaB ras 1. The immunogen is located within amino acids 80 - 130 of KappaB ras.
- Isotype
- IgG
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- Application Notes
-
KappaB ras antibody can be used for detection of KappaB ras by Western blot at 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 1 μ,g/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- KappaB ras Antibody is supplied in PBS containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,4 °C
- Storage Comment
- KappaB ras antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
-
- Target
- KappaB Ras
- Background
- KappaB ras Antibody: KappaB ras-1 (κ,B-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with Iκ,B (Iκ,B), an inhibitor of the transcription factor NF-κ,B. Iκ,B exists in two homologous forms, Iκ,B-alpha and Iκ,B-beta, although Iκ,B-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive Iκ,B-alpha-NF-κ,B complexes can shuttle in and out of the nucleus, Iκ,B-beta-NF-κ,B complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the Iκ,B-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of Iκ,B-beta and regulating the rate of degradation of Iκ,B-beta. This antibody detects both kappaB-ras1 and kappaB-ras2.
- Gene ID
- 28512
- UniProt
- Q9NYS0
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