HTRA2 antibody (C-Term)

Catalog No. ABIN6990409

This Rabbit Polyclonal antibody specifically detects HTRA2 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for HTRA2 antibody (C-Term) (ABIN6990409)

Target: HTRA2 (HtrA Serine Peptidase 2 (HTRA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTRA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HTRA2 Antibody

Binding Specificity: AA 70-120, C-Term

Purification: OMI Antibody is Ion exchange chromatography purified.

Immunogen: OMI antibody was raised against a peptide corresponding to 16 amino acids near the C-terminus of human Omi. The immunogen is located within amino acids 70 - 120 of OMI.

Isotype: IgG

Application Details

Application Notes: OMI antibody can be used for detection of OMI by Western blot at 0.5 to 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: OMI Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: OMI antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for HTRA2

Target: HTRA2 (HtrA Serine Peptidase 2 (HTRA2))

Alternative Name: OMI

Background: OMI Antibody: Inhibitor of apoptosis proteins (IAPs) were initially identified in baculoviruses as proteins that inhibit apoptosis of the host cells to allow time for viral replication. Cellular homologues containing at least one baculoviral IAP repeat (BIR) motif essential for their anti-apoptosis activity have been identified in yeasts and higher organisms and often act by binding and inhibiting processed caspases. The activity of these proteins can be modulated by the expression of proteins such as Smac/DIABLO and XAF-1 which displace or prevent the binding of caspases by IAPs. Recently, a mitochondrial serine protease termed Omi/HtrA2 has been found to bind IAPs. Similar to Smac, Omi possesses a conserved IAP-binding motif, but acts to cleave IAPs to irreversibly inactivate IAPs and promote apoptosis.

Gene ID: 27429

UniProt: O43464

Pathways: Positive Regulation of Endopeptidase Activity