PAK6 antibody (AA 260-310)

Catalog No. ABIN6990412

This Rabbit Polyclonal antibody specifically detects PAK6 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for PAK6 antibody (AA 260-310) (ABIN6990412)

Target: PAK6 (P21-Activated Kinase 6 (PAK6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PAK6 Antibody

Binding Specificity: AA 260-310

Specificity: PAK6 antibody is predicted to not cross-react with other PAK family proteins.

Purification: PAK6 Antibody is affinity chromatography purified via peptide column.

Immunogen: PAK6 antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK6. The immunogen is located within amino acids 260 - 310 of PAK6.

Isotype: IgG

Application Details

Application Notes: PAK6 antibody can be used for the detection of PAK6 by Western blot at 1 - 4 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PAK6 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PAK6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PAK6

Target: PAK6 (P21-Activated Kinase 6 (PAK6))

Alternative Name: PAK6

Background: PAK6 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK6 was initially identified as an androgen receptor in a yeast two hybrid screen and was found to be highly expressed in testis and prostate tissues. Later experiments have shown it to be activated by MAP kinase kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.

Molecular Weight: Predicted: 75 kDa

Observed: 70 kDa

Gene ID: 56924

NCBI Accession: NP_064553

UniProt: Q9NQU5