BCL2L14 antibody (N-Term)

Catalog No. ABIN6990432

This anti-BCL2L14 antibody is a Rabbit Polyclonal antibody detecting BCL2L14 in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for BCL2L14 antibody (N-Term) (ABIN6990432)

Target: BCL2L14 (BCL2-Like 14 (Apoptosis Facilitator) (BCL2L14))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL2L14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BCL2L14 Antibody

Binding Specificity: N-Term

Specificity: Although antibody should react with both isoforms, only the Bcl-GS protein has been observed

Purification: Bcl-G Antibody is Ion exchange chromatography purified.

Immunogen: Bcl-G antibody was raised against a peptide corresponding to 15 amino acids near the N-terminus of human Bcl-G. The immunogen is located within the first 50 amino acids of Bcl-G.

Isotype: IgG

Application Details

Application Notes: Bcl-G antibody can be used for detection of Bcl-G by Western blot at 2.5 to 5 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Bcl-G Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Bcl-G antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for BCL2L14

Target: BCL2L14 (BCL2-Like 14 (Apoptosis Facilitator) (BCL2L14))

Alternative Name: Bcl-G

Background: Bcl-G Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.

Gene ID: 79370

NCBI Accession: NM_030766

UniProt: Q9BZR8