XAF1 antibody (C-Term)

Catalog No. ABIN6990444

This anti-XAF1 antibody is a Rabbit Polyclonal antibody detecting XAF1 in WB, ELISA, IHC (p) and IF. Suitable for Human and Mouse.

Quick Overview for XAF1 antibody (C-Term) (ABIN6990444)

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XAF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-XAF1 Antibody

Binding Specificity: C-Term

Purification: XAF-1 Antibody is affinity chromatography purified via peptide column.

Immunogen: XAF-1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human XAF-1. The immunogen is located within the last 50 amino acids of XAF-1.

Isotype: IgG

Application Details

Application Notes: XAF-1 antibody can be used for the detection of XAF-1 by Western blot at 0.5 to 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: XAF-1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: XAF-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for XAF1

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Alternative Name: XAF-1

Background: XAF-1 Antibody: XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.

Molecular Weight: Predicted: 35 kDa

Observed: 32 kDa

Gene ID: 54739

UniProt: Q6GPH4