TRIM5 antibody (Middle Region)

Catalog No. ABIN6990447

This anti-TRIM5 antibody is a Rabbit Polyclonal antibody detecting TRIM5 in WB, ELISA, IHC (p) and IF. Suitable for Human.

Quick Overview for TRIM5 antibody (Middle Region) (ABIN6990447)

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-TRIM5 Antibody

Binding Specificity: AA 370-420, Middle Region

Purification: TRIM5 alpha Antibody is affinity chromatography purified via peptide column.

Immunogen: TRIM5 alpha antibody was raised against a synthetic peptide corresponding to amino acids near the mid-region of human TRIM5 alpha. The immunogen is located within amino acids 370 - 420 of TRIM5 alpha.

Isotype: IgG

Application Details

Application Notes: TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 2 μ,g/mL. A band at approximately 55 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TRIM5 alpha Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TRIM5 alpha antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TRIM5

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Alternative Name: TRIM5 alpha

Background: TRIM5 alpha Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α, variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5α, variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5α, acts as a natural barrier to cross-species retrovirus transmission.

Gene ID: 85363

NCBI Accession: NP_149023

UniProt: Q9C035

Pathways: Activation of Innate immune Response