TRIM5 antibody (AA 390-440)

Catalog No. ABIN6990449

Product Details anti-TRIM5 Antibody

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Binding Specificity: AA 390-440

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: TRIM5 alpha antibody will not cross-react with other isoforms of TRIM5.

Purification: TRIM5 alpha Antibody is affinity chromatography purified via peptide column.

Immunogen: TRIM5 alpha antibody was raised against a synthetic peptide corresponding to 19 amino acids near the center of rhesus monkey TRIM5 alpha. The immunogen is located within amino acids 390 - 440 of TRIM5 alpha.

Isotype: IgG

Application Details

Application Notes: TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TRIM5 alpha Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TRIM5 alpha antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TRIM5

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Alternative Name: TRIM5 alpha (TRIM5 Products)

Background: TRIM5 alpha Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α, variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5α, variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5α, acts as a natural barrier to cross-species retrovirus transmission.

Molecular Weight: Predicted: 54 kDa

Observed: 54 kDa

Gene ID: 574288

UniProt: Q0PF16

Pathways: Activation of Innate immune Response