Caspase 3 antibody (AA 70-120)

Catalog No. ABIN6990512

The Rabbit Polyclonal anti-Caspase 3 antibody has been validated for ELISA and IHC (p). It is suitable to detect Caspase 3 in samples from Human.

Quick Overview for Caspase 3 antibody (AA 70-120) (ABIN6990512)

Target: Caspase 3 (CASP3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Caspase 3 Antibody

Binding Specificity: AA 70-120

Specificity: At least two isoforms of Caspase-3 are known to exist.

Purification: Caspase-3 Antibody is affinity chromatography purified via peptide column.

Immunogen: Caspase-3 antibody was raised against a 17 amino acid synthetic peptide near the center of human Caspase-3. The immunogen is located within amino acids 70 - 120 of Caspase-3.

Isotype: IgG

Application Details

Application Notes: Caspase-3 antibody can be used for detection of Caspase-3 by immunohistochemistry at 5 μ,g/mL.

Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Caspase-3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Caspase-3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Caspase-3

Background: Caspase-3 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-3 is synthesized as an inactive proenzyme that undergoes proteolytic cleavage by caspases 8, 9 and 10 to produce 2 subunits, termed p20 and p11. These subunits dimerize to form the active enzyme. Caspase-3 proteolytically cleaves and activates other proteins such as caspases 6, 7 and 9.

Molecular Weight: 30 kDa

Gene ID: 836

NCBI Accession: NP_004337

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation