RPS6K1 (AA 600-650), (C-Term) antibody
Reactivity: Human
ICC, ELISA, WB, IF
Host: Rabbit
Polyclonal
unconjugated
-
- Target
- RPS6K1
- Binding Specificity
- AA 600-650, C-Term
- Reactivity
- Human
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunocytochemistry (ICC), ELISA, Western Blotting (WB), Immunofluorescence (IF)
- Purification
- RPS6K1 Antibody is affinity chromatography purified via peptide column.
- Immunogen
- RPS6K1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human RPS6K1. The immunogen is located within amino acids 600 - 650 of RPS6K1.
- Isotype
- IgG
-
- Application Notes
-
RPS6K1antibody can be used for the detection of RPS6K1 by Western blot at 2.5 - 10 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- RPS6K1 Antibody is supplied in PBS containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,4 °C
- Storage Comment
- RPS6K1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
-
- Target
- RPS6K1
- Background
- RPS6K1 Antibody: Ribosomal protein S6 kinase 1 (RPS6K1) is the best characterized effector of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Nutrients and growth factors stimulate a complex including TOR, raptor (regulatory associated protein of TOR), and GbetaL to phosphorylate RPS6K1 and the eukaryotic initiation factor 4E binding protein (4EBP1), leading to increased protein synthesis and cell growth. RPS6K1 is thought to desensitize tissues to insulin as mice deficient in RPS6K1 have been shown to be hypersensitive to insulin and impervious to obesity-induced insulin resistance seen in wild type obese mice.
- Gene ID
- 6195
- UniProt
- Q15418
-
You are here:
