RPS6K1 (AA 600-650), (C-Term) antibody

Catalog No. ABIN6990539

The Rabbit Polyclonal anti-RPS6K1 antibody is suitable to detect RPS6K1 in samples from Human. It has been validated for ICC, ELISA, WB and IF.

Quick Overview for RPS6K1 (AA 600-650), (C-Term) antibody (ABIN6990539)

Target: RPS6K1

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: Immunocytochemistry (ICC), ELISA, Western Blotting (WB), Immunofluorescence (IF)

Product Details

Binding Specificity: AA 600-650, C-Term

Purification: RPS6K1 Antibody is affinity chromatography purified via peptide column.

Immunogen: RPS6K1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human RPS6K1. The immunogen is located within amino acids 600 - 650 of RPS6K1.

Isotype: IgG

Application Details

Application Notes: RPS6K1antibody can be used for the detection of RPS6K1 by Western blot at 2.5 - 10 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: RPS6K1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: RPS6K1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details

Target: RPS6K1

Background: RPS6K1 Antibody: Ribosomal protein S6 kinase 1 (RPS6K1) is the best characterized effector of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Nutrients and growth factors stimulate a complex including TOR, raptor (regulatory associated protein of TOR), and GbetaL to phosphorylate RPS6K1 and the eukaryotic initiation factor 4E binding protein (4EBP1), leading to increased protein synthesis and cell growth. RPS6K1 is thought to desensitize tissues to insulin as mice deficient in RPS6K1 have been shown to be hypersensitive to insulin and impervious to obesity-induced insulin resistance seen in wild type obese mice.

Gene ID: 6195

UniProt: Q15418