Claudin 1 antibody (C-Term)

Catalog No. ABIN6990551

The Rabbit Polyclonal anti-Claudin 1 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect Claudin 1 in samples from Human and Rat.

Quick Overview for Claudin 1 antibody (C-Term) (ABIN6990551)

Target: Claudin 1 (CLDN1)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Claudin 1 Antibody

Binding Specificity: C-Term

Purification: CLDN1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-CLDN1 antibody was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of human CLDN1. The immunogen is located within the last 50 amino acids of CLDN1.

Isotype: IgG

Application Details

Application Notes: WB: 1-2 μ,g/mL, ICC: 5 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human and rat samples, Immunocytochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: CLDN1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: CLDN1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Claudin 1

Target: Claudin 1 (CLDN1)

Alternative Name: CLDN1

Background: CLDN1 Antibody: Claudin1 (CLDN1), a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Tight junctions are specialized regions of cell to cell contact consisting of networking strands that act as a molecular gasket for preventing the leakage of ions, water, etc., between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition and CLDN1 expression is often cell type and tissue dependent. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. CLDN1 and CLDN2 were found to be overexpressed in colonal cancer tissues and may be useful as tumor markers and targets for the treatment of colorectal cancer. Characterization of Claudins expression in human tumors can be an additional diagnostic tool. Recent studies show that CLDN1 has gastric tumor suppressive activity and is a direct transcriptional target of RUNX3. Along with SCARB1, LDL-R, and the tetraspanin superfamily member CD81, CLDN1 has been reported to be an entry factor for the Hepatitis C virus.

Molecular Weight: Predicted: 23kD

Observed: 23 kD kDa

Gene ID: 9076

UniProt: O95832

Pathways: Cell-Cell Junction Organization, Hepatitis C