LAMP2 antibody (C-Term)

Catalog No. ABIN6990579

This anti-LAMP2 antibody is a Rabbit Polyclonal antibody detecting LAMP2 in WB, ELISA, ICC and IHC (p). Suitable for Human and Mouse.

Quick Overview for LAMP2 antibody (C-Term) (ABIN6990579)

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAMP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LAMP2 Antibody

Binding Specificity: C-Term

Specificity: LAMP-2 antibody cross reacts with LAMP2B and LAMP2C, but not LAMP2A.

Purification: LAMP-2 Antibody is affinity chromatography purified via peptide column.

Immunogen: LAMP-2 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human LAMP-2. The immunogen is located within the last 50 amino acids of LAMP-2.

Isotype: IgG

Application Details

Application Notes: LAMP-2 antibody can be used for the detection of LAMP-2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL and immunohistochemistry starting at 5 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunocytochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: LAMP-2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: LAMP-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for LAMP2

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Alternative Name: LAMP-2

Background: LAMP-2 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). LAMP-2, a highly glycosylated protein associated with the lysosome, has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon's disease is associated with the accumulation of autophagic material in striated myocytes. LAMP-2 exists in multiple isoforms.

Gene ID: 16784

NCBI Accession: NP_054701

UniProt: P17047

Pathways: Autophagy