GOPC antibody (C-Term)

Catalog No. ABIN6990581

The Rabbit Polyclonal anti-GOPC antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect GOPC in samples from Human, Mouse and Rat.

Quick Overview for GOPC antibody (C-Term) (ABIN6990581)

Target: GOPC (Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GOPC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GOPC Antibody

Binding Specificity: AA 360-410, C-Term

Purification: PIST Antibody is affinity chromatography purified via peptide column.

Immunogen: PIST antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human PIST. The immunogen is located within amino acids 360 - 410 of PIST.

Isotype: IgG

Application Details

Application Notes: PIST antibody can be used for the detection of PIST by Western blot at 1 - 4 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in human and rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PIST Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PIST antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for GOPC

Target: GOPC (Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC))

Alternative Name: PIST

Background: PIST Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.

Gene ID: 57120

UniProt: Q9HD26

Pathways: Maintenance of Protein Location, Asymmetric Protein Localization