DDX3X antibody (AA 460-510)

Catalog No. ABIN6990618

This anti-DDX3X antibody is a Rabbit Polyclonal antibody detecting DDX3X in WB, ELISA and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for DDX3X antibody (AA 460-510) (ABIN6990618)

Target: DDX3X (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX3X antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-DDX3X Antibody

Binding Specificity: AA 460-510

Specificity: DDX3 antibody will detect both DDX3 and DBY.

Purification: DDX3 Antibody is affinity chromatography purified via peptide column.

Immunogen: DDX3 antibody was raised against a 16 amino acid synthetic peptide from near the center of human DDX3. The immunogen is located within amino acids 460 - 510 of DDX3.

Isotype: IgG

Application Details

Application Notes: DDX3 antibody can be used for the detection of DDX3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: DDX3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: DDX3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for DDX3X

Target: DDX3X (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X))

Alternative Name: DDX3

Background: DDX3 Antibody: DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.

Gene ID: 1654

UniProt: O00571

Pathways: Ribonucleoprotein Complex Subunit Organization, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Ribosome Assembly