IL-27 antibody (AA 80-130)

Catalog No. ABIN6990634

This Rabbit Polyclonal antibody specifically detects IL-27 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for IL-27 antibody (AA 80-130) (ABIN6990634)

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-27 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IL-27 Antibody

Binding Specificity: AA 80-130

Purification: IL-27 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-IL-27 antibody was raised against a peptide corresponding to 14 amino acids near the center of human IL-27. The immunogen is located within amino acids 80 - 130 of IL-27.

Isotype: IgG

Application Details

Application Notes: WB: 2-4 μ,g/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: IL-27 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: IL-27 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for IL-27

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Alternative Name: IL-27

Background: IL-27 Antibody: Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naive but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet.

Molecular Weight: Predicted: 28 kDa

Observed: 28 kDa

Gene ID: 246778

NCBI Accession: NP_663634

UniProt: Q8NEV9