Cyclin O antibody (AA 90-140)

Catalog No. ABIN6990652

Product Details anti-Cyclin O Antibody

Target: Cyclin O (CCNO)

Binding Specificity: AA 90-140

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin O antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Specificity: At least two isoforms of Cyclin O are known to exist, this antibody will recognize both isoforms.

Purification: Cyclin O Antibody is affinity chromatography purified via peptide column.

Immunogen: Cyclin O antibody was raised against a 13 amino acid synthetic peptide from near the center of human Cyclin O. The immunogen is located within amino acids 90 - 140 of Cyclin O.

Isotype: IgG

Application Details

Application Notes: Cyclin O antibody can be used for the detection of Cyclin O by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Cyclin O Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Cyclin O antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Cyclin O

Target: Cyclin O (CCNO)

Alternative Name: Cyclin O (CCNO Products)

Background: Cyclin O Antibody: Cyclin O, also known as CCNO, has recently been identified as a Cdk1- and Cdk2-activating cyclin specific to apoptosis in lymphoid cells. Cyclin O binds to and activates Cdk2 in response to instrinsic apoptotic stimuli such as glucocorticoids or DNA-damaging agents. Supression of Cyclin O expression by shRNA leads to the inhibition of glucocorticoid and DNA-damage-induced apoptosis due to a failure of apical caspase activation while leaving the CD95 death receptor-mediated apoptosis intact. Note: this gene, which had a previous symbol of UNG2, was erroneously identified as a uracil DNA glycosylase. A later publication identified this gene's product as a cyclin protein family member.

Molecular Weight: 39 kDa

Gene ID: 10309

NCBI Accession: NP_066970

UniProt: P22674