Cyclin O antibody

Catalog No. ABIN498417

The Rabbit Polyclonal anti-Cyclin O antibody (ABIN498417) specifically detects Cyclin O in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Cyclin O antibody (ABIN498417)

Target: Cyclin O (CCNO)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin O antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cyclin O Antibody

Specificity: This antibody detects endogenous levels of UDG protein. (region surrounding His221)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Cyclin O

Target: Cyclin O (CCNO)

Alternative Name: Uracil-DNA Glycosylase 2 / Cyclin-O

Background: The human UNG-gene encodes nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase using differentially regulated promoters, PA and PB, and alternative splicing to produce two proteins with unique N-terminal sorting sequences. Uracil in DNA may result from deamination of cytosine which can give rise to transition mutations. Uracil-DNA glycosylase is the DNA repair enzyme responsible for the removal of uracil from DNA. This is probably a biologically important function in the prevention of mutagenesis resulting from cytosine deamination. UNG2 removes misincorporated dUMP residues.Synonyms: CCNO, Cyclin-like uracil-DNA glycosylase, UDG2, UNG2

Molecular Weight: approx. 35 kDa

Gene ID: 10309

NCBI Accession: NP_066970

UniProt: P22674