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LIPE antibody (C-Term)

This Rabbit Polyclonal antibody specifically detects LIPE in WB, ELISA, IHC (p) and IF. It exhibits reactivity toward Human, Mouse and Rat.
Catalog No. ABIN6990685

Quick Overview for LIPE antibody (C-Term) (ABIN6990685)

Target

See all LIPE Antibodies
LIPE (Lipase, Hormone-Sensitive (LIPE))

Reactivity

  • 70
  • 51
  • 50
  • 2
  • 1
Human, Mouse, Rat

Host

  • 84
  • 5
  • 5
  • 1
Rabbit

Clonality

  • 81
  • 14
Polyclonal

Conjugate

  • 54
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  • 3
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  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
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  • 1
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This LIPE antibody is un-conjugated

Application

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  • 15
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  • 4
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  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
  • Binding Specificity

    • 15
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    • 9
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    • 2
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    • 1
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    • 1
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    • 1
    • 1
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    • 1
    • 1
    AA 920-970, C-Term

    Specificity

    Multiple isoforms of Lipe are known to exist.

    Purification

    Lipe Antibody is affinity chromatography purified via peptide column.

    Immunogen

    Lipe antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Lipe. The immunogen is located within amino acids 920 - 970 of Lipe.

    Isotype

    IgG
  • Application Notes

    Lipe antibody can be used for detection of Lipe by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

    Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Lipe Antibody is supplied in PBS containing 0.02 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C,4 °C

    Storage Comment

    Lipe antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Target

    LIPE (Lipase, Hormone-Sensitive (LIPE))

    Alternative Name

    Lipe

    Background

    Lipe Antibody: Although initially described as an adipocyte-specific triacylglycerol lipase, Lipe (also known as hormone-specific lipase or HSL) is expressed in multiple tissues and cell lines. It plays multiple roles in lipid metabolism, including hormone-stimulated lipolysis in adipose tissue and the hydrolysis of cholesterol esters. Lipe is expressed as a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesterol esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. Recently, it was observed that the lack of Lipe in genetically obese leptin-null mice inhibited obesity and adipogenesis, suggesting that Lipe plays a major role in adipocyte proliferation.

    Molecular Weight

    Predicted: 85, 90, 110, 118 kDa

    Observed: 90 kDa

    Gene ID

    3991

    UniProt

    Q05469

    Pathways

    AMPK Signaling, Monocarboxylic Acid Catabolic Process, Lipid Metabolism
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