PRDM1 antibody (C-Term)

Catalog No. ABIN6990694

This Rabbit Polyclonal antibody specifically detects PRDM1 in WB, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PRDM1 antibody (C-Term) (ABIN6990694)

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRDM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-PRDM1 Antibody

Binding Specificity: C-Term

Purification: Blimp-1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Blimp-1 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human Blimp-1. The immunogen is located within the last 50 amino acids of Blimp-1.

Isotype: IgG

Application Details

Application Notes: Blimp-1 antibody can be used for detection of Blimp-1 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunoflourescence starting at 20 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Blimp-1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Blimp-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PRDM1

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Alternative Name: Blimp-1

Background: Blimp-1 Antibody: Blimp-1 was initially identified as a zinc finger-containing protein that drives the maturation of B lymphocytes into immunoglobulin-secreting cells. Together with X-box-binding protein 1 (XBP1), Blimp-1 is induced upon terminal differentiation of plasma cells. The transcriptional repressor activity of Blimp-1 has also been found to regulate T cell homeostasis and function, possibly by suppressing the expression of the cytokines IL-2 and interferon-gamma during T cell development. More recent experiments have suggested that Blimp-1 also plays a major role in the formation of primordial germ cells (PGC) in developing mammalian embryos. In these experiments, Blimp-1-deficient mutant mouse embryos form a cluster of PGC-like cells which fail to show the expected migration, proliferation, and repression of homeobox genes that normally accompany specification of primordial germ cells.

Gene ID: 639

NCBI Accession: NP_001189

UniProt: O75626

Pathways: Regulation of Muscle Cell Differentiation