Klotho antibody (AA 410-460)

Catalog No. ABIN6990712

Product Details anti-Klotho Antibody

Target: Klotho (KL)

Binding Specificity: AA 410-460

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Klotho antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: Three isoforms of KLOTHO are known to exist.

Purification: KLOTHO Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-KLOTHO antibody was raised against a peptide corresponding to 17 amino acids near the center of human KLOTHO. The immunogen is located within amino acids 410-460 of KLOTHO.

Isotype: IgG

Application Details

Application Notes: WB: 1-4 μ,g/mL, IHC: 2.5 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in mouse and rat samples, Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: KLOTHO Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: KLOTHO antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Klotho

Target: Klotho (KL)

Alternative Name: KLOTHO (KL Products)

Background: KLOTHO Antibody: KLOTHO is the systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein that is abundant in the kidney and brain. In mice, a deficiency in KLOTHO expression leads to various systemic phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy together with growth retardation, short life-span and infertility. Transgenic mice overexpressing KLOTHO have an extended life span by inhibiting insulin/IGF1 signaling. KLOTHO is involved in the regulation of calcium/phosphorus homeostasis by inhibiting the synthesis of active vitamin D and identified as a potential tumor suppressor.

Molecular Weight: Predicted: 116kD

Observed: 116kD kDa

Gene ID: 9365

NCBI Accession: NP_004786

UniProt: Q9UEF7

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Activity, Growth Factor Binding