NPAS3 antibody (AA 470-520)

Catalog No. ABIN6990741

This anti-NPAS3 antibody is a Rabbit Polyclonal antibody detecting NPAS3 in WB, ELISA, IHC (p) and IF. Suitable for Human, Mouse and Rat.

Quick Overview for NPAS3 antibody (AA 470-520) (ABIN6990741)

Target: NPAS3 (Neuronal PAS Domain Protein 3 (NPAS3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPAS3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-NPAS3 Antibody

Binding Specificity: AA 470-520

Purification: NPAS3 Antibody is affinity chromatography purified via peptide column.

Immunogen: NPAS3 antibody was raised against a 28 amino acid synthetic peptide from near the center of human NPAS3. The immunogen is located within amino acids 470 - 520 of NPAS3.

Isotype: IgG

Application Details

Application Notes: NPAS3 antibody can be used for detection of NPAS3 by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: NPAS3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: NPAS3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for NPAS3

Target: NPAS3 (Neuronal PAS Domain Protein 3 (NPAS3))

Alternative Name: NPAS3

Background: NPAS3 Antibody: Neuronal PAS domain protein 3 (NPAS3) is a brain-enriched basic helix-loop-helix PAS domain transcription factor and is broadly expressed in the developing neuroepithelium and has recently found to be disrupted by genetic translocation in a family affected with schizophrenia. It was recently shown to be involved in the regulation of FGF signaling in the dentate gyrus by controlling the expression of the FGF receptor subtype 1 and in turn neurogenesis emanating from this region. NPAS3-null mice were growth-retarded and displayed brain defects that included reduced size of the anterior hippocampus, hypoplasia of the corpus callosum, and enlargement of the ventricles, as well as several behavioral abnormalities. Furthermore, these NPAS3-null mice also exhibited disruptions in several neurosignaling pathways involving glutamate, dopamine, and serotonin. These results demonstrate the essential role played by NPAS3 during structural and functional brain development. At least three isoforms of NPAS3 are known to exist.

Gene ID: 64067

UniProt: Q8IXF0