FYB antibody (N-Term)

Catalog No. ABIN6990773

The Rabbit Polyclonal anti-FYB antibody has been validated for WB, ELISA, ICC and IF. It is suitable to detect FYB in samples from Human.

Quick Overview for FYB antibody (N-Term) (ABIN6990773)

Target: FYB (FYN-Binding Protein (FYB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FYB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-FYB Antibody

Binding Specificity: AA 60-110, N-Term

Purification: ADAP Antibody is affinity chromatography purified via peptide column.

Immunogen: ADAP antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ADAP. The immunogen is located within amino acids 60 - 110 of ADAP.

Isotype: IgG

Application Details

Application Notes: ADAP antibody can be used for detection of ADAP by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ADAP Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ADAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for FYB

Target: FYB (FYN-Binding Protein (FYB))

Alternative Name: ADAP

Background: ADAP Antibody: The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-κ,B family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.

Gene ID: 2533

NCBI Accession: NP_001456

UniProt: O15117

Pathways: TCR Signaling, Protein targeting to Nucleus