KIF5A antibody (AA 330-380)

Catalog No. ABIN6990779

The Rabbit Polyclonal anti-KIF5A antibody has been validated for WB, ICC, ELISA and IF. It is suitable to detect KIF5A in samples from Human, Mouse and Rat.

Quick Overview for KIF5A antibody (AA 330-380) (ABIN6990779)

Target: KIF5A (Kinesin Family Member 5A (KIF5A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF5A antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunofluorescence (IF)

Product Details anti-KIF5A Antibody

Binding Specificity: AA 330-380

Specificity: KIF5 has at least three known isoforms, this antibody recognizes all three.

Purification: KIF5 Antibody is affinity chromatography purified via peptide column.

Immunogen: KIF5 antibody was raised against a 20 amino acid synthetic peptide from near the center of human KIF5. The immunogen is located within amino acids 330 - 380 of KIF5.

Isotype: IgG

Application Details

Application Notes: KIF5 antibody can be used for detection of KIF5 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: KIF5 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: KIF5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for KIF5A

Target: KIF5A (Kinesin Family Member 5A (KIF5A))

Alternative Name: KIF5

Background: KIF5 Antibody: The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. Mutations in one of the three isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.

Molecular Weight: Predicted: 105, 114 kDa

Observed: 118 kDa

Gene ID: 3798

UniProt: Q12840