TRIM25 antibody (C-Term)

Catalog No. ABIN6990789

This anti-TRIM25 antibody is a Rabbit Polyclonal antibody detecting TRIM25 in WB, ELISA, IF and ICC. Suitable for Human and Mouse.

Quick Overview for TRIM25 antibody (C-Term) (ABIN6990789)

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-TRIM25 Antibody

Binding Specificity: AA 520-570, C-Term

Purification: TRIM25 Antibody is affinity chromatography purified via peptide column.

Immunogen: TRIM25 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human TRIM25. The immunogen is located within amino acids 520 - 570 of TRIM25.

Isotype: IgG

Application Details

Application Notes: TRIM25 antibody can be used for detection of TRIM25 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 20 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TRIM25 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TRIM25 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TRIM25

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Alternative Name: TRIM25

Background: TRIM25 Antibody: TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.

Gene ID: 7706

NCBI Accession: NP_005073

UniProt: Q14258