Endonuclease G antibody (N-Term)

Catalog No. ABIN6990798

This anti-Endonuclease G antibody is a Rabbit Polyclonal antibody detecting Endonuclease G in WB, ELISA, IHC (p) and IF. Suitable for Human, Rat and Mouse.

Quick Overview for Endonuclease G antibody (N-Term) (ABIN6990798)

Target: Endonuclease G (ENDOG)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endonuclease G antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-Endonuclease G Antibody

Binding Specificity: AA 40-90, N-Term

Purification: EndoG Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-EndoG antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human ENDOG. The immunogen is located within amino acids 40-90 of EndoG.

Isotype: IgG

Application Details

Application Notes: WB: 0.5-2 μ,g/mL, IF: 20 μ,g/mL, IHC: 2.5-15 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: EndoG Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: EndoG antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Endonuclease G

Target: Endonuclease G (ENDOG)

Alternative Name: EndoG

Background: EndoG Antibody: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndooG also cleaves DNA in vitro.

Molecular Weight: Predicted: 33kD

Observed: 33kD kDa

Gene ID: 2021

NCBI Accession: NP_004426

UniProt: Q14249

Pathways: Apoptosis