CD248 antibody (N-Term)

Catalog No. ABIN6990809

The Rabbit Polyclonal anti-CD248 antibody has been validated for WB, ELISA, IHC (p) and IF. It is suitable to detect CD248 in samples from Human.

Quick Overview for CD248 antibody (N-Term) (ABIN6990809)

Target: CD248 (CD248 Molecule, Endosialin (CD248))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD248 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-CD248 Antibody

Binding Specificity: AA 40-90, N-Term

Specificity: At least two isoforms of TEM1 are known to exist, this antibody recognizes only the larger isoform.

Purification: TEM1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-TEM-1 antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human TEM-1. The immunogen is located within amino acids 40-90 of TEM-1

Isotype: IgG

Application Details

Application Notes: WB: 0.5-2 μ,g/mL, IHC-P: 2.5 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TEM1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TEM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CD248

Target: CD248 (CD248 Molecule, Endosialin (CD248))

Alternative Name: TEM1

Background: TEM1 Antibody: Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion.

Molecular Weight: Predicted: 81kD

Observed: 90 kD (post-modifications: 27 O-linked glycosylations) kDa

Gene ID: 57124

NCBI Accession: NP_065137

UniProt: Q9HCU0