ZBP1 antibody (C-Term)

Catalog No. ABIN6990827

The Rabbit Polyclonal anti-ZBP1 antibody has been validated for WB, ELISA, IHC (p) and IF. It is suitable to detect ZBP1 in samples from Human, Mouse and Rat.

Quick Overview for ZBP1 antibody (C-Term) (ABIN6990827)

Target: ZBP1 (Z-DNA Binding Protein 1 (ZBP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-ZBP1 Antibody

Binding Specificity: C-Term

Specificity: Multiple isoforms of ZBP1 are known to exist.

Purification: ZBP1 Antibody is affinity chromatography purified via peptide column.

Immunogen: ZBP1 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human ZBP1. The immunogen is located within the last 50 amino acids of ZBP1.

Isotype: IgG

Application Details

Application Notes: ZBP1 antibody can be used for the detection of ZBP1 by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ZBP1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ZBP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ZBP1

Target: ZBP1 (Z-DNA Binding Protein 1 (ZBP1))

Alternative Name: ZBP1

Background: ZBP1 Antibody: Z-DNA binding protein 1 (ZBP1) belongs to a family of proteins that contain the Zalpha domain which binds specifically to left-handed Z-DNA and Z-RNA. ZBP1 was initially identified as a novel gene that was up-regulated in activated macrophages in mice bearing ascites tumors, suggesting that is may play a role in processes such as host response in neoplasia. More recent reports indicate that the cytosolic ZBP1 has can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of ZBP1 to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK).

Molecular Weight: Predicted: 39, 45 kDa

Observed: 42 kDa

Gene ID: 81030

UniProt: Q9H171