DDIT4 antibody (AA 90-140)

Catalog No. ABIN6990867

This anti-DDIT4 antibody is a Rabbit Polyclonal antibody detecting DDIT4 in WB, ELISA, IHC (p) and IF. Suitable for Human, Rat and Mouse.

Quick Overview for DDIT4 antibody (AA 90-140) (ABIN6990867)

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDIT4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-DDIT4 Antibody

Binding Specificity: AA 90-140

Purification: RTP801 Antibody is affinity chromatography purified via peptide column.

Immunogen: RTP801 antibody was raised against a 12 amino acid synthetic peptide from near the center of human RTP801. The immunogen is located within amino acids 90 - 140 of RTP801.

Isotype: IgG

Application Details

Application Notes: RTP801 antibody can be used for the detection of RTP801 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: RTP801 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: RTP801 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for DDIT4

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Alternative Name: RTP801

Background: RTP801 Antibody: RTP801 was initially identified as a gene induced by DNA damage, and later found to also be regulated by other cellular stresses such as hypoxia and glucocorticoid treatment. Recently, RTP801 has been shown to act as a mediator of tuberous sclerosis complex (TSC)-dependent regulation of the mammalian Target of Rapamycin (mTOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle. In response to energy stress, RTP801 inhibits mTOR function, resulting in dephosphorylation of downstream targets such as ribosomal protein S6 kinase 1 and 4EBP1 and decreasing cell growth. Disregulation of RTP801 may thus contribute to human tumorigenesis.

Gene ID: 54541

NCBI Accession: NP_061931

UniProt: Q9NX09

Pathways: Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process