Syntaphilin antibody (AA 350-400)

Catalog No. ABIN6990920

The Rabbit Polyclonal anti-Syntaphilin antibody is suitable to detect Syntaphilin in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC (p) and IF.

Quick Overview for Syntaphilin antibody (AA 350-400) (ABIN6990920)

Target: Syntaphilin (SNPH)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Syntaphilin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-Syntaphilin Antibody

Binding Specificity: AA 350-400

Purification: Syntaphilin Antibody is affinity chromatography purified via peptide column.

Immunogen: Syntaphilin antibody was raised against a 16 amino acid synthetic peptide from near the center of human Syntaphilin. The immunogen is located within amino acids 350 - 400 of Syntaphilin.

Isotype: IgG

Application Details

Application Notes: Syntaphilin antibody can be used for detection of Syntaphilin by Western blot at 1 - 2 μ,g/mL. Despite its predicted molecular weight, Syntaphilin usually migrates at higher molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Syntaphilin Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Syntaphilin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Syntaphilin

Target: Syntaphilin (SNPH)

Alternative Name: Syntaphilin

Background: Syntaphilin Antibody: Syntaphilin was initially identified in a yeast two-hybrid screen with the carboxy terminal region of Syntaxin-1 as bait. Syntaxin-1 is a key component of the synaptic vesicle docking machinery that forms the SNARE complex with synaptobrevin and SNAP-25. Syntaphilin competes with SNAP-25 for binding to syntaxin-1 and inhibits the formation of the SNARE complex, thereby potentially regulating synaptic vesicle exocytosis. Syntaphilin also binds dynamin-1 and inhibits dynamin-dependent endocytosis. Mice lacking syntaphilin show an increased level of mitochondrial motility and a reduced density of axonal mitochondria. This correlates with an enhanced short-term facilitation and significant impairments in motor ability, suggesting syntaphilin plays a major role in presynaptic function. Multiple isoforms are known to exist.

Gene ID: 9751

UniProt: O15079

Pathways: Synaptic Vesicle Exocytosis