S1PR1 antibody (C-Term)

Catalog No. ABIN6990983

The Rabbit Polyclonal anti-S1PR1 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect S1PR1 in samples from Human, Mouse and Rat.

Quick Overview for S1PR1 antibody (C-Term) (ABIN6990983)

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-S1PR1 Antibody

Binding Specificity: C-Term

Specificity: At least two isoforms of S1P1 are known to exist, this S1P1 antibody will only recognize the shorter isoform.

Purification: S1P1 Antibody is affinity chromatography purified via peptide column.

Immunogen: S1P1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of the human S1P1. The immunogen is located within the last 50 amino acids of S1P1.

Isotype: IgG

Application Details

Application Notes: S1P1 antibody can be used for detection of S1P1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: S1P1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: S1P1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for S1PR1

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Alternative Name: S1P1

Background: S1P1 Antibody: Movement of lymphocytes through lymphoid organs is required for generating immunity. Their migration into lymph nodes follows a series of events including integrin activation through chemokine signaling, adhesion and diapedis. The release of lymphocytes from lymph nodes is regulated by the phospholipid sphingosine-1-phosphate (S1P). One of its receptors S1P1 binds S1P with high specificity and affinity, agonism of this receptor by the immunosuppressive agent FTY720 inhibits the entry of tissue T cells into afferent lymphatics in homeostatic and inflammatory conditions. Recent experiments have indicated that CCR7-deficient T cells left lymph nodes more rapidly than wild-type cells did and these cells where also less effectively retained after treatment with FTY720, and that egress competence could be restored by inactivating G alpha i-protein-coupled receptor signaling. These results suggest that S1P1 acts in the lymphocyte to promote lymph node egress by overcoming retention signals mediated by CCR7 and G alpha i-protein-coupled receptor signaling.

Molecular Weight: Predicted: 42 kDa

Observed: 42 kDa

Gene ID: 1901

NCBI Accession: NP_001391

UniProt: P21453

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2