AAK1 antibody (C-Term)

Catalog No. ABIN6990990

This anti-AAK1 antibody is a Rabbit Polyclonal antibody detecting AAK1 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for AAK1 antibody (C-Term) (ABIN6990990)

Target: AAK1 (AP2 associated kinase 1 (AAK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AAK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-AAK1 Antibody

Binding Specificity: AA 830-880, C-Term

Purification: Aak1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Aak1 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of the human Aak1. The immunogen is located within amino acids 830 - 880 of Aak1.

Isotype: IgG

Application Details

Application Notes: Aak1 antibody can be used for detection of Aak1 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Aak1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Aak1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for AAK1

Target: AAK1 (AP2 associated kinase 1 (AAK1))

Alternative Name: Aak1

Background: Aak1 Antibody: AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxphi) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist, the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.

Gene ID: 22848

NCBI Accession: NP_055726

UniProt: Q2M2I8