SPRED1 antibody (AA 120-170)

Catalog No. ABIN6990991

This Rabbit Polyclonal antibody specifically detects SPRED1 in WB, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SPRED1 antibody (AA 120-170) (ABIN6990991)

Target: SPRED1 (Sprouty-Related, EVH1 Domain Containing 1 (SPRED1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRED1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SPRED1 Antibody

Binding Specificity: AA 120-170

Purification: Spred1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Spred1 antibody was raised against a 20 amino acid synthetic peptide near the center of the human Spred1. The immunogen is located within amino acids 120 - 170 of Spred1.

Isotype: IgG

Application Details

Application Notes: Spred1 antibody can be used for detection of Spred1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Spred1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Spred1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for SPRED1

Target: SPRED1 (Sprouty-Related, EVH1 Domain Containing 1 (SPRED1))

Alternative Name: Spred1

Background: Spred1 Antibody: The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes, it is inhibited by Spred1, a member of the Sprouty family of proteins. Spred1 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). This Spred1 antibody is predicted to have no cross-reactivity to Spred2 or Spred3.

Gene ID: 161742

NCBI Accession: NP_689807

UniProt: Q7Z699

Pathways: Positive Regulation of Response to DNA Damage Stimulus