DACT2 antibody (N-Term)

Catalog No. ABIN6991002

Product Details anti-DACT2 Antibody

Target: DACT2 (Dapper, Antagonist of beta-Catenin 2 (DACT2))

Binding Specificity: AA 150-200, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DACT2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: Dact2 Antibody is affinity chromatography purified via peptide column.

Immunogen: Dact2 antibody was raised against a 12 amino acid synthetic peptide from near the amino terminus of human DACT2. The immunogen is located within amino acids 150 - 200 of Dact2.

Isotype: IgG

Application Details

Application Notes: Dact2 antibody can be used for detection of Dact2 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Dact2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Dact2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for DACT2

Target: DACT2 (Dapper, Antagonist of beta-Catenin 2 (DACT2))

Alternative Name: Dact2 (DACT2 Products)

Synonyms: 2900084M21Rik antibody, A630024E20 antibody, Dpr2 antibody, Frd2 antibody, dapper2 antibody, C6orf116 antibody, DAPPER2 antibody, DPR2 antibody, RP11-503C24.7 antibody, bA503C24.7 antibody, dishevelled binding antagonist of beta catenin 2 antibody, dishevelled-binding antagonist of beta-catenin 2 antibody, DACT2 antibody, Dact2 antibody

Background: Dact2 Antibody: The Wnt signaling cascade is a conserved process in multicellular animals that plays important roles during development and can contribute to cancer and other diseases. Many members of this pathway are also expressed in the postnatal tissues such as brain. One such protein is Dact2, a member of the Dact protein family that was initially identified through binding to Disheveled (Dvl), a cytoplasmic protein essential to Wnt signaling. Dact2 is most prominent during the development of the thymus kidneys, and salivary gland. Dact2 is thought to play a role distinct from that of Dact1 with Dact2 having a greater impact on a beta-catenin-independent process termed planar cell polarity/convergent-extension signaling. Furthermore, Dact2 but not Dact1 can inhibit Nodal signaling by promoting the endocytic degradation of TGF-beta receptors. At least two isoforms of Dact2 are known to exist.

Gene ID: 168002

NCBI Accession: NP_999627

UniProt: Q5SW24