RSPO1 antibody (N-Term)

Catalog No. ABIN6991124

The Rabbit Polyclonal anti-RSPO1 antibody has been validated for WB, ELISA, IHC (p) and IF. It is suitable to detect RSPO1 in samples from Human and Mouse.

Quick Overview for RSPO1 antibody (N-Term) (ABIN6991124)

Target: RSPO1 (R-Spondin 1 (RSPO1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RSPO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-RSPO1 Antibody

Binding Specificity: N-Term

Specificity: At least three isoforms of RSPO1 are known to exist, this antibody will detect the largest and smallest isoforms. RSPO1 antibody will not cross-react with other RSPO family members.

Purification: RSPO1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-RSPO1 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human RSPO1. The immunogen is located within the first 50 amino acids of RSPO1.

Isotype: IgG

Application Details

Application Notes: WB: 0.5-2 μ,g/mL, IHC: 2.5-5 μ,g/mL, IF: 5 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: RSPO1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: RSPO1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for RSPO1

Target: RSPO1 (R-Spondin 1 (RSPO1))

Alternative Name: RSPO1

Background: RSPO1 Antibody: RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular beta-catenin, thereby regulating functions mediated by beta-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels.

Molecular Weight: Predicted: 29kD

Observed: 36 kD (Post-modification: 1 N-linked glycosylation) kDa

Gene ID: 284654

NCBI Accession: NP_001033722

UniProt: Q2MKA7