UCMA antibody (C-Term)

Catalog No. ABIN6991131

Product Details anti-UCMA Antibody

Target: UCMA (Upper Zone of Growth Plate and Cartilage Matrix Associated (UCMA))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCMA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: UCMA Antibody is affinity chromatography purified via peptide column.

Immunogen: UCMA antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human UCMA. The immunogen is located within the last 50 amino acids of UCMA.

Isotype: IgG

Application Details

Application Notes: UCMA antibody can be used for detection of UCMA by Western blot at 2.5 - 5 μ,g/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: UCMA Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: UCMA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for UCMA

Target: UCMA (Upper Zone of Growth Plate and Cartilage Matrix Associated (UCMA))

Alternative Name: UCMA (UCMA Products)

Background: UCMA Antibody: UCMA is a secreted cartilage-specific protein that was discovered in a screen for differentially expressed genes in retinoic acid-treated mouse chondrocytes. It was also identified in a human chondrocyte EST screen for candidate genes of skeletal dysplasias. UCMA expression is thought to parallel that of collagen II with its expression decreasing with maturation chrondrocytes mature. UCMA is processed by a furin-like protease into two fragments, an amino-terminal fragment and a carboxy-terminal fragment (UCMA-C). Application of recombinant UCMA-C to primary osteoblasts, mesenchymal stem cells, and MC3T3-E1 pre-osteoblasts interferes with their osteogenic differentiation, but does not affect expression of chondrocyte-specific genes or chondrocyte proliferation, suggesting that UCMA may be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells. At least two isoforms of UCMA are known to exist.

Molecular Weight: Predicted: 15 kDa

Observed: 19 kDa

Gene ID: 221044

NCBI Accession: NP_660357

UniProt: Q8WVF2