NOTUM antibody (N-Term)

Catalog No. ABIN6991166

The Rabbit Polyclonal anti-NOTUM antibody is suitable to detect NOTUM in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC (p) and IF.

Quick Overview for NOTUM antibody (N-Term) (ABIN6991166)

Target: NOTUM (Notum Pectinacetylesterase Homolog (NOTUM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOTUM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-NOTUM Antibody

Binding Specificity: AA 90-140, N-Term

Purification: NOTUM Antibody is affinity chromatography purified via peptide column.

Immunogen: NOTUM antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NOTUM. The immunogen is located within amino acids 90 - 140 of NOTUM.

Isotype: IgG

Application Details

Application Notes: NOTUM antibody can be used for detection of NOTUM by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: NOTUM Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: NOTUM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for NOTUM

Target: NOTUM (Notum Pectinacetylesterase Homolog (NOTUM))

Alternative Name: NOTUM

Background: NOTUM Antibody: In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by beta-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.

Gene ID: 147111

NCBI Accession: NP_848588

UniProt: Q6P988