PICALM antibody (N-Term)

Catalog No. ABIN6991249

This Rabbit Polyclonal antibody specifically detects PICALM in WB, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PICALM antibody (N-Term) (ABIN6991249)

Target: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PICALM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-PICALM Antibody

Binding Specificity: N-Term

Purification: PICALM Antibody is affinity chromatography purified via peptide column.

Immunogen: PICALM antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human PICALM. The immunogen is located within the first 50 amino acids of PICALM.

Isotype: IgG

Application Details

Application Notes: PICALM antibody can be used for detection of PICALM by Western blot at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PICALM Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PICALM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PICALM

Target: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Alternative Name: PICALM

Background: PICALM Antibody: PICALM, also known as the clathrin assembly lymphoid myeloid leukemia (CALM) protein, co-localizes with clathrin at clathrin-coated areas of the plasma membrane and in the Golgi area, and is thought to be an important component of the coated pit internalization machinery. Fusion proteins involving PICALM have been implicated in acute myeloid and T-cell acute lymphoblastic leukemia. PICALM is also expressed in neurons and is present at the synapse where it facilitates the endocytosis of the synaptic vesicle protein VAMP2. Recent studies have identified variants of PICALM associated with Alzheimer's disease and have suggested this protein may play a role in the endocytosis and recycling of the amyloid protein precursor (APP).

Gene ID: 8301

NCBI Accession: NP_009097

UniProt: Q13492

Pathways: Synaptic Membrane