EFHD2 antibody (N-Term)

Catalog No. ABIN6991298

The Rabbit Polyclonal anti-EFHD2 antibody has been validated for ELISA, WB, IHC (p) and IF. It is suitable to detect EFHD2 in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for EFHD2 antibody (N-Term) (ABIN6991298)

Target: EFHD2 (EF-Hand Domain Family, Member D2 (EFHD2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EFHD2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-EFHD2 Antibody

Binding Specificity: N-Term

Purification: EFHD2 Antibody is affinity chromatography purified via peptide column.

Immunogen: EFHD2 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human EFHD2. The immunogen is located within the first 50 amino acids of EFHD2.

Isotype: IgG

Application Details

Application Notes: EFHD2 antibody can be used for detection of EFHD2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: EFHD2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: EFHD2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

References for anti-EFHD2 antibody (ABIN6991298)

ODonnell, Dagley, Curley, Darbey, OShaughnessy, Diemer, Pilatz, Fietz, Stanton, Smith, Rebourcet: "Sertoli cell-enriched proteins in mouse and human testicular interstitial fluid." in: PloS one, Vol. 18, Issue 9, pp. e0290846, (2023) (PubMed).

Target Details for EFHD2

Target: EFHD2 (EF-Hand Domain Family, Member D2 (EFHD2))

Alternative Name: EFHD2

Background: EFHD2 Antibody: EFHD2, also known as Swiprosin-1 or SWS1, is an EF-hand and coiled-coil-containing adaptor protein that plays a role in lymphocyte physiology. EFHD2 exhibits the highest expression in CD8+ T cells and immature B cells. It provides a membrane scaffold that is required for the Syk-, SLP-65-, and PLCgamma2-dependent B-cell receptor (BCR)-induced calcium flux. EFHD2 may also regulate BCR-induced immature and primary B-cell apoptosis. It controls spontaneous apoptosis through the regulation of BCL2L1 abundance. Also, EFHD2 plays a role as negative regulator of the canonical NF-κ,B-activating branch.

Gene ID: 79180

NCBI Accession: NP_077305

UniProt: Q96C19